The spike protein of murine coronavirus mouse hepatitis virus strain A59 is not cleaved in primary glial cells and primary hepatocytes.
Identifieur interne : 006498 ( Main/Exploration ); précédent : 006497; suivant : 006499The spike protein of murine coronavirus mouse hepatitis virus strain A59 is not cleaved in primary glial cells and primary hepatocytes.
Auteurs : S T Hingley [États-Unis] ; I. Leparc-Goffart ; S R WeissSource :
- Journal of virology [ 0022-538X ] ; 1998.
Descripteurs français
- KwdFr :
- MESH :
- physiologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus de l'hépatite murine.
- virologie : Foie, Névroglie.
- Animaux, Glycoprotéine de spicule des coronavirus, Réplication virale, Souris.
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : Membrane Glycoproteins, Viral Envelope Proteins.
- physiology : Murine hepatitis virus.
- virology : Liver, Neuroglia.
- Animals, Mice, Spike Glycoprotein, Coronavirus, Virus Replication.
Abstract
Mouse hepatitis virus strain A59 (MHV-A59) produces meningoencephalitis and severe hepatitis during acute infection. Infection of primary cells derived from the central nervous system (CNS) and liver was examined to analyze the interaction of virus with individual cell types derived from the two principal sites of viral replication in vivo. In glial cell cultures derived from C57BL/6 mice, MHV-A59 produces a productive but nonlytic infection, with no evidence of cell-to-cell fusion. In contrast, in continuously cultured cells, this virus produces a lytic infection with extensive formation of syncytia. The observation of few and delayed syncytia following MHV-A59 infection of hepatocytes more closely resembles infection of glial cells than that of continuously cultured cell lines. For MHV-A59, lack of syncytium formation correlates with lack of cleavage of the fusion glycoprotein, or spike (S) protein. The absence of cell-to-cell fusion following infection of both primary cell types prompted us to examine the cleavage of the spike protein. Cleavage of S protein was below the level of detection by Western blot analysis in MHV-A59-infected hepatocytes and glial cells. Furthermore, no cleavage of this protein was detected in liver homogenates from C57BL/6 mice infected with MHV-A59. Thus, cleavage of the spike protein does not seem to be essential for entry and spread of the virus in vivo, as well as for replication in vitro.
PubMed: 9445064
Affiliations:
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Leparc-Goffart</name></author><author><name sortKey="Weiss, S R" sort="Weiss, S R" uniqKey="Weiss S" first="S R" last="Weiss">S R Weiss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:9445064</idno><idno type="pmid">9445064</idno><idno type="wicri:Area/PubMed/Corpus">003492</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003492</idno><idno type="wicri:Area/PubMed/Curation">003492</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003492</idno><idno type="wicri:Area/PubMed/Checkpoint">003371</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003371</idno><idno type="wicri:Area/Ncbi/Merge">003C61</idno><idno type="wicri:Area/Ncbi/Curation">003C61</idno><idno type="wicri:Area/Ncbi/Checkpoint">003C61</idno><idno type="wicri:doubleKey">0022-538X:1998:Hingley S:the:spike:protein</idno><idno type="wicri:Area/Main/Merge">006A13</idno><idno type="wicri:Area/Main/Curation">006498</idno><idno type="wicri:Area/Main/Exploration">006498</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The spike protein of murine coronavirus mouse hepatitis virus strain A59 is not cleaved in primary glial cells and primary hepatocytes.</title><author><name sortKey="Hingley, S T" sort="Hingley, S T" uniqKey="Hingley S" first="S T" last="Hingley">S T Hingley</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, Philadelphia College of Osteopathic Medicine, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Philadelphia College of Osteopathic Medicine, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Leparc Goffart, I" sort="Leparc Goffart, I" uniqKey="Leparc Goffart I" first="I" last="Leparc-Goffart">I. Leparc-Goffart</name></author><author><name sortKey="Weiss, S R" sort="Weiss, S R" uniqKey="Weiss S" first="S R" last="Weiss">S R Weiss</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Liver (virology)</term><term>Membrane Glycoproteins (physiology)</term><term>Mice</term><term>Murine hepatitis virus (physiology)</term><term>Neuroglia (virology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (physiology)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Foie (virologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (physiologie)</term><term>Névroglie (virologie)</term><term>Protéines de l'enveloppe virale (physiologie)</term><term>Réplication virale</term><term>Souris</term><term>Virus de l'hépatite murine (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Foie</term><term>Névroglie</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Liver</term><term>Neuroglia</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Mice</term><term>Spike Glycoprotein, Coronavirus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Réplication virale</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mouse hepatitis virus strain A59 (MHV-A59) produces meningoencephalitis and severe hepatitis during acute infection. Infection of primary cells derived from the central nervous system (CNS) and liver was examined to analyze the interaction of virus with individual cell types derived from the two principal sites of viral replication in vivo. In glial cell cultures derived from C57BL/6 mice, MHV-A59 produces a productive but nonlytic infection, with no evidence of cell-to-cell fusion. In contrast, in continuously cultured cells, this virus produces a lytic infection with extensive formation of syncytia. The observation of few and delayed syncytia following MHV-A59 infection of hepatocytes more closely resembles infection of glial cells than that of continuously cultured cell lines. For MHV-A59, lack of syncytium formation correlates with lack of cleavage of the fusion glycoprotein, or spike (S) protein. The absence of cell-to-cell fusion following infection of both primary cell types prompted us to examine the cleavage of the spike protein. Cleavage of S protein was below the level of detection by Western blot analysis in MHV-A59-infected hepatocytes and glial cells. Furthermore, no cleavage of this protein was detected in liver homogenates from C57BL/6 mice infected with MHV-A59. Thus, cleavage of the spike protein does not seem to be essential for entry and spread of the virus in vivo, as well as for replication in vitro.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region></list><tree><noCountry><name sortKey="Leparc Goffart, I" sort="Leparc Goffart, I" uniqKey="Leparc Goffart I" first="I" last="Leparc-Goffart">I. Leparc-Goffart</name><name sortKey="Weiss, S R" sort="Weiss, S R" uniqKey="Weiss S" first="S R" last="Weiss">S R Weiss</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Hingley, S T" sort="Hingley, S T" uniqKey="Hingley S" first="S T" last="Hingley">S T Hingley</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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